Automated Comet Assay Imaging and Dual-Mask Analysis to Determine DNA Damage on an Individual Comet Basis
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چکیده
Mammalian cells incur DNA damage and alterations as a result of internal factors, such as stress, environment, oxidation and metabolism, as well as exposure to external stimuli, such as chemical compounds, UV and cosmic radiation. Genotoxicity studies aim to identify and understand the mechanisms of DNA damage and repair, and also to seek methods of pathway regulation and dysregulation for improved therapeutic success. Beyond therapeutic implications, a number of industries, including cosmetics, food/beverage, chemical and mining/ oil/petroleum, incorporate genotoxicity testing to characterize the safety profile of their products.
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